The bill establishes regulations for intercollegiate athletic competitions at the University of Wyoming and Wyoming community colleges, mandating that students compete based on their biological sex as recorded on their official birth certificates. It defines key terms such as "athletic competition" and "educational institution," and prohibits educational institutions from allowing students to compete in competitions designated for the opposite sex. However, it does allow female students to compete in male-designated competitions if no corresponding female competition is available.

Additionally, the bill includes provisions to protect individuals who report violations, stating that educational institutions cannot retaliate against them. It also allows for disciplinary action against those who make false reports and provides a civil cause of action for individuals against educational institutions for violations, with a cap on liability set at $50,000. The University of Wyoming and the Wyoming community college commission are tasked with creating rules to implement these regulations, ensuring compliance with confidentiality laws regarding student medical information. The act is set to take effect on July 1, 2025.