The bill establishes definitions and standards for the application of a person's biological sex in Wyoming law, emphasizing the distinction between males and females. It defines "sex" as a person's biological sex at birth and provides specific definitions for "male," "female," "mother," and "father." The bill also clarifies that separate accommodations for males and females are not inherently unequal and mandates that individuals born with certain medical conditions receive legal protections under the Americans with Disabilities Act. Additionally, it states that laws distinguishing between sexes in contexts such as athletics, prisons, and shelters are permissible if they serve important governmental objectives related to health, safety, and privacy.

Furthermore, the bill requires that any school district or state agency collecting vital statistics must identify individuals as either male or female based on their sex at birth. This provision aims to ensure accurate data collection for public health and anti-discrimination purposes. The act is set to take effect immediately upon the completion of necessary legislative procedures.