The bill establishes definitions and standards for the application of a person's biological sex in Wyoming law, emphasizing the distinction between males and females. It defines "sex" as a person's biological sex at birth, with specific definitions for "male," "female," "woman," "girl," "man," "boy," "mother," and "father." The bill also clarifies that separate accommodations for males and females are not inherently unequal and mandates that individuals born with a recognized condition of "disorder or difference in sex development" receive legal protections under the Americans with Disabilities Act.

Additionally, the bill requires that any school district or state agency collecting vital statistics must identify individuals as either male or female based on their sex at birth. It asserts that laws distinguishing between sexes in contexts such as athletics, prisons, and shelters are justified by important governmental objectives related to health, safety, and privacy. The act is set to take effect immediately upon completion of the necessary legislative processes.