The bill establishes a new section in Wyoming law that explicitly prohibits the jurisdiction of the United States Centers for Disease Control and Prevention (CDC) and the World Health Organization (WHO) within the state. It states that any requirements, mandates, recommendations, instructions, or guidance from these organizations cannot be used to justify mask, vaccine, or medical testing requirements in Wyoming, rendering such directives without force or effect in the state.

The act is set to take effect on July 1, 2023, reinforcing the state's stance on public health autonomy by limiting the influence of specified federal and nongovernmental health organizations on local health mandates and requirements.