House Bill 1539, also known as Senate Bill 2113, amends Tennessee law to establish regulations regarding the possession and use of nitrous oxide, commonly referred to as "laughing gas." The bill introduces a new part to Tennessee Code Annotated, Title 39, Chapter 17, making it an offense to knowingly inhale, ingest, use, or possess nitrous oxide or amyl nitrite, with violations classified as a Class A misdemeanor. Additionally, the trial court may require individuals convicted under this section to participate in a drug rehabilitation program as a condition of probation.

The bill outlines specific exceptions to this prohibition, including the use of nitrous oxide when prescribed by a licensed medical or dental practitioner, in manufacturing processes, for food preparation, or for automotive purposes, provided the individual is at least 21 years old. It also establishes a rebuttable presumption against these exceptions if the substance contains additional flavoring or is labeled as flavored. Furthermore, the bill imposes penalties on businesses and entities found in violation, including a 30-day suspension of their operating licenses for a first offense and revocation for subsequent violations. The act is set to take effect on July 1, 2026.