The "Protection of Women's Sports Act" aims to ensure equal athletic opportunities for males and females in public educational institutions and private athletic clubs. The bill mandates that all athletic teams, competitions, and events be designated specifically for males, females, or as coeducational. It prohibits male participation in teams or events designated for females, while allowing males to serve as practice players under certain conditions, provided they do not take opportunities away from female athletes. The act also establishes definitions for key terms such as "athletic association," "team," "male," "female," and "sex," emphasizing the biological classification of individuals.

Additionally, the bill provides a framework for compliance and enforcement, stating that no adverse actions can be taken against institutions that adhere to its provisions. It allows individuals who are harmed by violations of the act to pursue private legal action for remedies, including injunctive relief and compensatory damages. The act aims to protect women's sports by ensuring that only biologically female athletes can compete in designated female events, thereby reinforcing the separation of athletic opportunities based on sex.