The bill, known as the Stand With Women Act, establishes regulations regarding interscholastic athletic teams based on sex in public and private educational institutions in Nebraska. It asserts that athletic teams must be designated as male, female, or coed, and prohibits males from participating in female-designated teams unless no female team is available. The legislation emphasizes the physical differences between males and females, arguing that these differences justify the need for separate teams to ensure safety and equal opportunities for female athletes. Additionally, it requires students to provide documentation confirming their sex to participate in sex-designated teams.

The bill also includes provisions that protect schools from complaints or investigations related to maintaining separate teams for female students and mandates that educational institutions adopt policies to implement the Act. Furthermore, it ensures that individuals with disorders or differences in sex development receive protections under the Americans with Disabilities Act. The Act includes a severability clause, ensuring that if any part is found unconstitutional, the remaining sections will still be valid.