The bill establishes honorary designations for various transportation facilities across Florida, directing the Department of Transportation to erect suitable markers for each designated area. Notable designations include the "Heroes Memorial Overpass" in Bradford County, the "Sergeant Elio Diaz Memorial Highway" in Charlotte County, and the "PBSO Motorman Highway" in Palm Beach County, among others. A total of 21 specific designations are outlined, honoring individuals such as law enforcement officers and public figures.

Additionally, the bill amends existing law by revising the designation of a portion of U.S. 27/S.R. 25 in Highlands County to "Deputy William Gentry, Jr., Memorial Highway," changing the description from the previous boundaries of the Polk County line and the Glades County line to a more specific location at "9200 U.S. 27 South and South George Boulevard." The effective date for the implementation of this act is set for July 1, 2025.